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HA-Tag Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

HA-Tag Monoclonal Antibody

貨號：

HYK-200061
規(guī)格：
圖片：
Western Blot
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 35, 48 kDa
Observed band size: 35, 35, 48 kDa

Western Blot
Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: HA-tagged fusion protein
All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
All lanes: HA-Tag antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution

Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

產(chǎn)品詳情

別名：

HA-Tag
宿主：

Mouse
反應(yīng)種屬：

All
免疫原：

YPYDVPDYA synthetic peptide conjugate to KLH
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2b
純化方式：

>95%, Protein A purified
克隆號：

18B11H6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF, IP, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:5000-1:160000
IF	1:50-1:200
IP	1μg-5μg
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.